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KMID : 0545120140240121679
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 12 p.1679 ~ p.1684
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Characterization of Acetobacter pomorum KJY8 Isolated from Korean Traditional Vinegar
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Baek Chang-Ho
Park Eun-Hee
Baek Seong-Yeol
Jeong Seok-Tae
Kim Myoung-Dong
Kwon Joong-Ho
Jeong Yong-Jin
Yeo Soo-Hwan
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Abstract
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Acetobacter sp. strains were isolated from traditional vinegar collected in Daegu city and Gyeongbuk province. The strain KJY8 showing a high acetic acid productivity was isolated and characterized by phenotypic, chemotaxonomic, and phylogenetic inference based on 16S rRNA sequence analysis. The chemotaxonomic and phylogenetic analyses revealed the isolate to be a strain of Acetobacter pomorum. The isolate showed a G+C content of 60.8 mol%. It contained LL-diaminopimelic acid (LL-A2pm) as the cell wall amino acid and ubiquinone Q9 (H6) as the major quinone. The predominant cellular fatty acids were C18:1w9c, w12t, and w7c. Strain KJY8 grew rapidly on glucose-yeast extract (GYC) agar and formed pale white colonies with smooth to rough surfaces. The optimum cultivation conditions for acetic acid production by the KJY8 strain were 20°C and pH 3.0, with an initial ethanol concentration of 9% (w/v) to produce an acetic acid concentration of 8% (w/v).
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KEYWORD
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Vinegar, Acetic acid bacteria, Acetobacter pomorum
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